Induction of a detoxification gene in Drosophila melanogaster requires an interaction between tissue specific enhancers and a novel cis-regulatory element
Henry Chung, Adrian Boey, Chris Lumb, Lee Willoughby, Philip Batterham, Phillip J Daborn
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2011
Organisms induce the expression of detoxification enzymes such as cytochrome P450s to deal with xenobiotics encountered in the environment. Research using cell culture systems has identified some of the cis-regulatory elements (CREs) and transcription factors involved in the induction of P450 genes in response to xenobiotic challenges. It was recently found that the CREs required for the basal expression of some P450s are distinct from the CREs involved in their induction. How these CREs mediate induction to xenobiotics in a tissue specific manner is not known. In this paper we show that, in Drosophila melanogaster, the induction response of the P450 gene Cyp6g1 to the xenobiotic Phenobarbit..View full abstract
We thank Greta Raymant for assistance with some fly work. The Australian Drosophila Biomedical Research Support Facility and Bloomington Drosophila Stock Center are acknowledged for providing Drosophila stocks. P.J.D. is supported by an Australian Research Council Australian Research Fellowship.