Journal article

An efficient high-throughput screening method for MYST family acetyltransferases, a new class of epigenetic drug targets

H Falk, T Connor, H Yang, KJ Loft, JL Alcindor, G Nikolakopoulos, RN Surjadi, JD Bentley, MK Hattarki, O Dolezal, JM Murphy, BJ Monahan, TS Peat, T Thomas, JB Baell, JP Parisot, IP Street

Journal of Biomolecular Screening | SAGE PUBLICATIONS INC | Published : 2011

Abstract

Epigenetic aberrations are increasingly regarded as key factors in cancer progression. Recently, deregulation of histone acetyltransferases (HATs) has been linked to several types of cancer. Monocytic leukemia zinc finger protein (MOZ) is a member of the MYST family of HATs, which regulate gene expression in cell proliferation and differentiation. Deregulation of these processes through constitutively active MOZ fusion proteins gives rise to the formation of leukemic stem cells, rendering MOZ an excellent target for treating myeloid leukemia. The authors implemented a hit discovery campaign to identify small-molecule inhibitors of MOZ-HAT activity. They developed a robust, homogeneous assay ..

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University of Melbourne Researchers