Phylogenetic analysis of rotavirus A NSP2 gene sequences and evidence of intragenic recombination
Nicole C Donker, Karen Boniface, Carl D Kirkwood
INFECTION GENETICS AND EVOLUTION | ELSEVIER SCIENCE BV | Published : 2011
The rotavirus non-structural protein NSP2 is one of the earliest and most abundant viral proteins produced during infection. This protein has multiple essential roles in the replication cycle involving RNA binding, viroplasm formation, helicase and can hydrolyse the γ-phosphate of RNA and NTPs acting as an RTPase and NTPase. In studying sequences from rotavirus strains isolated in Australia between 1984 and 2009, the NSP2 gene was seen to be highly conserved and clustered with defined NSP2 genotypes N1 and N2 according to the full genome based rotavirus classification system. Phylogenetic analysis indicated that NSP2 gene sequences isolated from Australian rotavirus strains formed four disti..View full abstract
Awarded by CDA
The authors thank Miss Jacqueline Williams for critical review of the manuscript. C.D. Kirkwood was supported by a CDA Research Fellowship for the National Health and Medical Research Council of Australia (609347). N.C. Donker was supported by an Australian Postgraduate Award Scholarship.