Fructose modulates cardiomyocyte excitation-contraction coupling and ca 2 handling in vitro

Abstract

Background: High dietary fructose has structural and metabolic cardiac impact, but the potential for fructose to exert direct myocardial action is uncertain. Cardiomyocyte functional responsiveness to fructose, and capacity to transport fructose has not been previously demonstrated. Objective: The aim of the present study was to seek evidence of fructose-induced modulation of cardiomyocyte excitation-contraction coupling in an acute, in vitro setting. Methods and Results: The functional effects of fructose on isolated adult rat cardiomyocyte contractility and Ca 2+ handling were evaluated under physiological conditions (37°C, 2 mM Ca 2+, HEPES buffer, 4 Hz stimulation) using video edge detec..