Journal article
Surface immobilization of bio-functionalized cubosomes: Sensing of proteins by quartz crystal microbalance
SJ Fraser, X Mulet, L Martin, S Praporski, A Mechler, PG Hartley, A Polyzos, F Separovic
Langmuir | AMER CHEMICAL SOC | Published : 2012
DOI: 10.1021/la2032994
Abstract
A strategy for tethering lipid liquid crystalline submicrometer particles (cubosomes) to a gold surface for the detection of proteins is reported. Time-resolved quartz crystal microbalance (QCM-D) was used to monitor the cubosome-protein interaction in real time. To achieve specific binding, cubosomes were prepared from the nonionic surfactant phytantriol, block-copolymer, Pluronic F-127, and a secondary biotinylated lipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethyleneglycol) -2000], which enabled attachment of the particles to a neutravidin (NAv)-alkanethiol monolayer at the gold surface of the QCM sensor chip. A second set of cubosomes was further functionalized ..
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Funding Acknowledgements
We thank CSIRO OCE PhD Scheme for funding (S.J.F.). We acknowledge Lynne Waddington for her valued help in obtaining the Cryo-TEM images. L.M. would like to acknowledge the financial support received from the Australian Research Council. S.J.F. would like to thank the David Lachlan Hay Memorial Fund (Postgraduate Writing-Up Award) for their generous financial support.