Engineered Bacterially Expressed Polypeptides: Assembly into Polymer Particles with Tailored Degradation Profiles
Denison HC Chang, Angus PR Johnston, Kim L Wark, Kerry Breheney, Frank Caruso
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | WILEY-V C H VERLAG GMBH | Published : 2012
In nature, the sequence of amino acids in a protein is determined by the genetic code. Biosynthesis of polypeptides by bacteria can be used to exploit this natural process to afford exact control over properties such as molecular weight, chemical functionality, and structure. It is demonstrated how control over the positioning of functional groups can be used to tune the degradation of assembled polypeptide particles (see scheme).
Awarded by Australian Research Council
This work was supported by the Australian Research Council under the Federation Fellowship (FF0776078) and Discovery Project (DP0877360) schemes. We thank Dr. Henk Dam for his assistance with mass spectrometry, Dr. Christopher J. Ochs and Dr. Zhiyuan Zhu for their assistance with NMR analysis, and Dr. Lillian Lee for her assistance with CD spectroscopy.