Journal article
RECK in osteosarcoma
Jonathan CM Clark, Toru Akiyama, David M Thomas, Agatha Labrinidis, Andreas Evdokiou, Stuart J Galloway, Han‐Soo Kim, Crispin R Dass, Peter FM Choong
Cancer | Wiley | Published : 2011
DOI: 10.1002/cncr.25757
Abstract
BACKGROUND: Targeted therapy in osteosarcoma (OS) is needed to improve patient outcomes. Human RECK may have a role because it inhibits cancer invasion and regulates angiogenesis. This study aimed to characterize RECK expression in human OS, to examine in vitro effects of RECK on vascular endothelium and OS cell behavior, and to analyze the effect of RECK on OS grown orthotopically in nude mice. METHODS: RECK was examined in human OS samples. Interactions between RECK and VEGF were studied in tissue and cells. RECK transfection was used to study its effects on vascular endothelial (HMEC-1) and OS (SaOS-2) cell behavior in vitro and in vivo. SaOS-2 co-culture with RAW 246.7-derived osteoclast..
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