Intestinal epithelial-specific PTEN inactivation results in tumor formation
Do-Sun Byun, Naseem Ahmed, Shannon Nasser, Joongho Shin, Sheren Al-Obaidi, Sanjay Goel, Georgia A Corner, Andrew J Wilson, Dustin J Flanagan, David S Williams, Leonard H Augenlicht, Elizabeth Vincan, John M Mariadason
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2011
Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a negative regulator of phosphatidylinositol 3-kinase (PI3K) signaling that is frequently inactivated in colorectal cancer through mutation, loss of heterozygosity, or epigenetic mechanisms. The aim of this study was to determine the effect of intestinal-specific PTEN inactivation on intestinal epithelial homeostasis and tumorigenesis. PTEN was deleted specifically in the intestinal epithelium, by crossing PTEN(Lox/Lox) mice with villin(Cre) mice. PTEN was robustly expressed in the intestinal epithelium and maximally in the differentiated cell compartment. Targeted inactivation of PTEN in the intestinal epithelium of PTEN(Lox/..View full abstract
Awarded by National Cancer Institute
Awarded by National Health and Medical Research Council of Australia
Awarded by NATIONAL CANCER INSTITUTE
These studies were supported by National Cancer Institute Grants CA-123316, CA-100823, and CA-88104 and National Health and Medical Research Council of Australia Project Grant ID 566679. J. M. Mariadason is supported by a Future Fellowship from the Australian Research Council.