Journal article
Subcellular localization of a fluorescent derivative of Cu II(atsm) offers insight into the neuroprotective action of Cu II(atsm)
KA Price, PJ Crouch, S Lim, BM Paterson, JR Liddell, PS Donnelly, AR White
Metallomics | ROYAL SOC CHEMISTRY | Published : 2011
DOI: 10.1039/c1mt00092f
Abstract
Copper complexes of bis(thiosemicarbazone) (Cu II(btsc)s) have been studied as potential anti-cancer agents and hypoxia imaging agents. More recently, Cu II(btsc)s have been identified as possessing potent neuroprotective properties in cell and animal models of neurodegenerative disease. Despite their broad range of pharmacological activity little is known about how cells traffic Cu II(btsc)s and how this relates to potential anti-cancer or neuroprotective outcomes. One method of investigating sub-cellular localization of metal complexes is through confocal fluorescence imaging of the compounds in cells. Previously we harnessed the fluorescence of a pyrene group attached to diacetyl-bis(N4-m..
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Funding Acknowledgements
This work was supported by funding from the National Health and Medical Research Council of Australia and the Australian Research Council of Australia. We would like to thank Prof. James Camakaris, Department of Genetics, The University of Melbourne and Dr James Hickey, School of Chemistry, The University of Melborurne for their valuable suggestions and input into this work and Mrs Anna Freidhuber, Department of Pathology, The University of Melbourne for help with the EM.