Journal article
Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites and identifies a novel component of the toxoplasma invasion motor complex
T Nebl, JH Prieto, E Kapp, BJ Smith, MJ Williams, JR Yates, AF Cowman, CJ Tonkin
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2011
Abstract
Apicomplexan parasites depend on the invasion of host cells for survival and proliferation. Calcium-dependent signaling pathways appear to be essential for micronemal release and gliding motility, yet the target of activated kinases remains largely unknown. We have characterized calcium-dependent phosphorylation events during Toxoplasma host cell invasion. Stimulation of live tachyzoites with Ca2+-mobilizing drugs leads to phosphorylation of numerous parasite proteins, as shown by differential 2-DE display of 32[P]-labeled protein extracts. Multi-dimensional Protein Identification Technology (MudPIT) identified ~546 phosphorylation sites on over 300 Toxoplasma proteins, including 10 sites on..
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Funding Acknowledgements
This work was funded by The National Health and Medical Research Council (NHMRC) of Australia. CJT is supported by an NHMRC Career Development Award. AFC is an NHMRC Australia Fellow. This work was also made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.