Journal article
Promiscuous peptide of 16 kDa antigen linked to Pam2Cys protects against mycobacterium tuberculosis by evoking enduring memory T-cell response
U Gowthaman, V Singh, W Zeng, S Jain, KF Siddiqui, SB Chodisetti, RK Gurram, P Parihar, P Gupta, UD Gupta, DC Jackson, JN Agrewala
Journal of Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2011
Abstract
One of the main reasons considered for BCG failure in tuberculosis-endemic areas is impediment by environmental mycobacteria in its processing and generation of memory T-cell response. To overcome this problem, we developed a unique lipopeptide (L91) by linking the promiscuous peptide (sequence 91-110) of 16 kDa antigen of Mycobacterium tuberculosis to Pam2Cys. L91 does not require extensive antigen processing and generates enduring Th1 memory response. This is evidenced by the fact that L91 significantly improved the activation, proliferation, and generation of protective T cells. Furthermore, L91 surmounts the barrier of major histocompatibility complex polymorphism and induces better prot..
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Funding Acknowledgements
[ "We thank Andrea Cooper, PhD, Trudeau Institute, New York, and Robert J. Wilkinson, PhD, Imperial College, London, for their critical reading of the manuscript. We thank Priya Gowthaman for helping with the manuscript preparation. We are grateful to V. M. Katoch, MD, NJIL&OMD, Agra, India, for providing the Biosafety Level 3 facility and to B. N. Dutta, MD, Medicos Centre, Chandigarh, India, for histopathological analysis. U. G. and K. F. S. are recipients of a DBT fellowship, and V. S., S. J, R. G., and S. C. are receipients of a CSIR fellowship.", "This work was supported by the Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology (DBT), New Delhi, India." ]