Journal article
Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles
ER Porrello, KDG Pfleger, RM Seeber, H Qian, C Oro, F Abogadie, LMD Delbridge, WG Thomas
Cellular Signalling | ELSEVIER SCIENCE INC | Published : 2011
Abstract
The cardiovascular hormone angiotensin II (AngII) exerts its actions via two G protein-coupled receptor (GPCR) subtypes, AT 1 and AT 2, which often display antagonistic functions. Methodological constraints have so far precluded detailed analyses of the ligand-dependency, cellular localization, and functional relevance of AngII receptor interactions in live cells. In this study, we utilize a protein-fragment complementation assay (PCA) and GPCR-Heteromer Identification Technology (GPCR-HIT) to provide the first detailed investigation of the ligand-dependency and cellular localization of AngII receptor interactions in human embryonic kidney 293 cells. Fluorescent-tagged receptor constructs fo..
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Awarded by Baker Foundation
Funding Acknowledgements
We thank Dr. Stephen Michnick (Universite de Montreal, Montreal, Canada) for provision of PCA plasmids and Dr. Clara Nahmias (Institut Cochin, Paris, France) for provision of ATIP plasmids. This work was supported by the National Health and Medical Research Council of Australia, the Australian Research Council, the National Heart Foundation of Australia and the Wenkart Foundation. E.R.P. was supported by an Australian Postgraduate Award and a Baker Foundation Postgraduate Award. K.D.G.P is supported by an Australian Research Council Future Fellowship (FT100100271). We gratefully acknowledge Dr. Nicola Smith for critical reading of this manuscript.