Journal article
ChIP-seq analysis reveals distinct H3K27me3 profiles that correlate with transcriptional activity
MD Young, TA Willson, MJ Wakefield, E Trounson, DJ Hilton, ME Blewitt, A Oshlack, IJ Majewski
Nucleic Acids Research | Published : 2011
DOI: 10.1093/nar/gkr416
Abstract
Transcriptional control is dependent on a vast network of epigenetic modifications. One epigenetic mark of particular interest is tri-methylation of lysine 27 on histone H3 (H3K27me3), which is catalysed and maintained by Polycomb Repressive Complex 2 (PRC2). Although this histone mark is studied widely, the precise relationship between its local pattern of enrichment and regulation of gene expression is currently unclear. We have used ChIP-seq to generate genome-wide maps of H3K27me3 enrichment, and have identified three enrichment profiles with distinct regulatory consequences. First, a broad domain of H3K27me3 enrichment across the body of genes corresponds to the canonical view of H3K27m..
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Awarded by Australian Research Council
Funding Acknowledgements
National Health and Medical Research Council (NHMRC) fellowship (406676 to M.E.B.); Australian Research Council QEII fellowship (DP1096092 to M.E.B.). I.J.M. funded by NHMRC grant 575581. Funding for open access charge: NHMRC grant 490037.