Journal article
GM-CSF increases cross-presentation and CD103 expression by mouse CD8 spleen dendritic cells
Y Zhan, EM Carrington, A van Nieuwenhuijze, S Bedoui, S Seah, Y Xu, N Wang, JD Mintern, JA Villadangos, IP Wicks, AM Lew
European Journal of Immunology | Published : 2011
Abstract
Resident CD8 + DCs perform several functions, including cross-presenting antigen and rapidly engulfing the Gram-positive intracellular pathogen Listeria monocytogenes. Little is known about how these functions of CD8 + DCs are modulated. Here, we show that granulocyte-macrophage CSF (GM-CSF), a cytokine that exists at low levels at steady state but is elevated during infection and inflammation, enhances cross-presentation and rapid uptake of L. monocytogenes by resident CD8 + DCs. This previously unrecognized functional enhancement of CD8 + DCs by GM-CSF was independent of promoting DC survival in vitro. Enhancement of these functions by GM-CSF was also marked by CD103 expression on CD8 + DC..
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by NHMRC Independent Research Institutes
Funding Acknowledgements
The authors thank the technical assistance from Nicole Ashman and Manny Hancock. This work was supported by the National Health and Medical Research Council of Australia (NH&MRC) program and project grants (#575543, #637324 and 1007703), Diabetes Australia, Juvenile Diabetes Research Foundation, NHMRC Independent Research Institutes Infrastructure Support Scheme grant #361646, and Victorian State Government Operational Infrastructure Support grant. Y. X. was supported by a Peter Doherty Fellowship from NH&MRC.