Seminiferous Cord Formation Is Regulated by Hedgehog Signaling in the Marsupial
Jin Wei Chung, Andrew J Pask, Marilyn B Renfree
BIOLOGY OF REPRODUCTION | SOC STUDY REPRODUCTION | Published : 2012
The signaling molecule DHH, secreted by Sertoli cells, has essential regulatory functions in testicular differentiation. DHH is required for the differentiation of peritubular myoid cells that line the seminiferous cords and steroidogenic Leydig cells. The testicular cords in Dhh-null male mice lack a basal lamina and develop abnormally. To date, the DHH-signaling pathway has never been examined outside of any eutherian mammals. This study examined the effects of inhibition of DHH signaling in a marsupial mammal, the tammar wallaby, by culturing gonads in vitro in the presence of the hedgehog-signaling inhibitors cyclopamine and forskolin. Disruption of hedgehog signaling in the tammar teste..View full abstract
Supported by a grant from the National Health and Medical Research Council (NHMRC) to M.B.R., A.J.P., and G.S.; the Australian Research Council (ARC) Centre of Excellence in Kangaroo Genomics, an R.D. Wright fellowship from the NHMRC, to A.J.P.; and an ARC Federation Fellowship to M.B.R.