Induction of Protective CD4( ) T Cell-Mediated Immunity by a Leishmania Peptide Delivered in Recombinant Influenza Viruses
Katherine Kedzierska, Joan M Curtis, Sophie A Valkenburg, Lauren A Hatton, Hiu Kiu, Peter C Doherty, Lukasz Kedzierski
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2012
The available evidence suggests that protective immunity to Leishmania is achieved by priming the CD4(+) Th1 response. Therefore, we utilised a reverse genetics strategy to generate influenza A viruses to deliver an immunogenic Leishmania peptide. The single, immunodominant Leishmania-specific LACK(158-173) CD4(+) peptide was engineered into the neuraminidase stalk of H1N1 and H3N2 influenza A viruses. These recombinant viruses were used to vaccinate susceptible BALB/c mice to determine whether the resultant LACK(158-173)-specific CD4(+) T cell responses protected against live L. major infection. We show that vaccination with influenza-LACK(158-173) triggers LACK(158-173)-specific Th1-biased..View full abstract
Awarded by Australian National Health and Medical Research Council
Awarded by NHMRC
This work was supported by the Australian National Health and Medical Research Council, (Project Grant 406631 and Program Grant 406601), NHMRC Independent Research Institutes Infrastructure Support Scheme grant #361646, and Victorian State Government Operational Infrastructure Support grant. KK is an NHMRC RD Wright Research Fellow. LAH and HK are recipients of an NHMRC Biomedical Postgraduate Research Scholarship, and SAV is a recipient of the Australian Postgraduate Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.