Journal article

In situ Biological Dose Mapping Estimates the Radiation Burden Delivered to 'Spared' Tissue between Synchrotron X-Ray Microbeam Radiotherapy Tracks

Kai Rothkamm, Jeffrey C Crosbie, Frances Daley, Sarah Bourne, Paul R Barber, Borivoj Vojnovic, Leonie Cann, Peter AW Rogers

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2012

Abstract

Microbeam radiation therapy (MRT) using high doses of synchrotron X-rays can destroy tumours in animal models whilst causing little damage to normal tissues. Determining the spatial distribution of radiation doses delivered during MRT at a microscopic scale is a major challenge. Film and semiconductor dosimetry as well as Monte Carlo methods struggle to provide accurate estimates of dose profiles and peak-to-valley dose ratios at the position of the targeted and traversed tissues whose biological responses determine treatment outcome. The purpose of this study was to utilise γ-H2AX immunostaining as a biodosimetric tool that enables in situ biological dose mapping within an irradiated tissue..

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Grants

Awarded by Cancer Research UK


Funding Acknowledgements

This study was funded by the Australian Nuclear Science & Technology Organisation, Cancer Council Victoria, National Health & Medical Research Council, Cancer Research UK, and the NIHR Centre for Research in Health Protection at the Health Protection Agency. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The views expressed in this publication are those of the authors and not necessarily those of the NHS, the National Institute for Health Research, or the Department of Health.