Journal article

Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection

Pavel Dolezal, Margareta Aili, Janette Tong, Jhih-Hang Jiang, Carlo M Marobbio, Sau Fung Lee, Ralf Schuelein, Simon Belluzzo, Eva Binova, Aurelie Mousnier, Gad Frankel, Giulia Giannuzzi, Ferdinando Palmieri, Kipros Gabriel, Thomas Naderer, Elizabeth L Hartland, Trevor Lithgow

PLoS Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2012

Abstract

The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionellanucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype tha..

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Grants

Awarded by Italian Human ProteomeNet


Awarded by Czech Science Foundation


Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by Medical Research Council


Funding Acknowledgements

This work was funded by grants from the Ministero dell'Universita e della Ricerca (MIUR), the Center of Excellence in Genomics (CEGBA), Fondazione Cassa di Risparmio di Puglia, the Italian Human ProteomeNet No. RBRN07BMCT_009 (MIUR), Czech Science Foundation grant P305/10/0651, Program Grant (606788) from the National Health and Medical Research Council (NHMRC) of Australia, the Australian Research Council (ARC) and the Wellcome Trust. MA was supported by a Wenner-Gren Foundation Fellowship, SB by an Australian Postgraduate Award and JHJ by an Endeavor Postgraduate Award. ELH is an ARC Future Fellow, TL is an ARC Federation Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.