Bismuth ions inhibit the biological activity of non-amidated gastrins in vivo

S Kovac; SW Loh; S Lachal; A Shulkes; GS Baldwin

Journal article

Bismuth ions inhibit the biological activity of non-amidated gastrins in vivo

S Kovac, SW Loh, S Lachal, A Shulkes, GS Baldwin

Biochemical Pharmacology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2012

DOI: 10.1016/j.bcp.2011.11.030

Abstract

The peptide hormone gastrin binds two ferric ions with high affinity, and iron binding is essential for the biological activity of non-amidated gastrins in vitro and in vivo. Bi 3+ ions also bind to glycine-extended gastrin17 (Ggly), but inhibit Ggly-induced cell proliferation and migration in gastrointestinal cell lines in vitro. The aims of the present study were firstly, to establish the mechanism by which Bi 3+ ions inhibit the binding of Fe 3+ ions to Ggly, and secondly, to test the effect of Bi 3+ ions on the activity of non-amidated gastrins in vivo. The interaction between Bi 3+ ions, Fe 3+ ions and Ggly was investigated by ultraviolet spectroscopy. The effect of Bi 3+ ions on colore..

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University of Melbourne Researchers

Arthur Shulkes Author

Related Projects (4)

RESEARCH FELLOWSHIP - PROF ARTHUR SHULKES

I am a physiologist with expertise in endocrinology determining the roles of particular regulatory peptides in the normal functions of the g..

Senior Principal Research Fellowship Renewal

I am a physiologist with expertise in endocrinology determining the roles of particular regulatory peptides in the normal functions of the g..

Gastrin, Iron and Colorectal Cancer

Our objective is to understand the biological significance of the interaction between iron and the hormone gastrin. The project's significan..

THE BIOLOGY OF GASTRIN-FERRIC ION COMPLEXES

The objective of this project is to understand the biological significance of the complex between ferric ions and non-amidated gastrins (NAG..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

We thank Chelsea Dumesny and Mildred Yim for skilled assistance with the mice and radioimmunoassays, respectively. This work was supported by grant 5 RO1 GM065926-08 from the National Institutes of Health (to GB, AS), grants 400062 and 566555 (to GB, AS), 208926 (to GB) and 350235 (to AS) from the National Health and Medical Research Council of Australia.