Foxp3 T cells in peripheral blood of renal transplant recipients and clinical correlations

WX Lin; D Christiansen; LL Fu; MA Roberts; MS Sandrin; FL Ierino

Journal article

Foxp3 T cells in peripheral blood of renal transplant recipients and clinical correlations

WX Lin, D Christiansen, LL Fu, MA Roberts, MS Sandrin, FL Ierino

Nephrology | WILEY | Published : 2012

DOI: 10.1111/j.1440-1797.2012.01578.x

Abstract

Aim: Immunophenotype peripheral blood T cells from renal transplant recipients (RTR) using cellular markers of regulatory T cells (Tregs) and flow cytometry, including Foxp3, and correlate these findings with clinical parameters. Methods: Expression of phenotypic markers of Tregs was assessed by flow cytometric analysis of peripheral blood lymphocytes (PBL) from (i) RTR (n = 95); (ii) patients with end-stage renal failure (ESRF) awaiting transplantation (n = 17); and (iii) normal healthy controls (n = 18). Results: The percentage of CD4 +CD25 +Foxp3 + cells within the CD4 + cell population did not significantly alter at different time points post-transplant. However, the percentage of CD4 +C..

View full abstract

University of Melbourne Researchers

Mauro Sandrin Author

Francesco Ierino Author

Grants

Funding Acknowledgements

The authors acknowledge the support from the following funding sources: Austin Hospital Medical Research Foundation (AHMRF), National Health Medical Research Council (NHMRC) and Philip Bladin Memorial Fund, Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne. The authors also thank Ms Bogumila Mioduszewski (Department of Nephrology, Austin Health, Heidelberg 3084, Victoria, Australia) for assistance with patient recruitment.