Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test

Abstract

This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions. Copyright © 2012, American Society for Microbiology. All Rights Reserved.