Drosophila Ribosomal Protein Mutants Control Tissue Growth Non-Autonomously via Effects on the Prothoracic Gland and Ecdysone
Jane I Lin, Naomi C Mitchell, Marina Kalcina, Elly Tchoubrieva, Mary J Stewart, Steven J Marygold, Cherryl D Walker, George Thomas, Sally J Leevers, Richard B Pearson, Leonie M Quinn, Ross D Hannan
PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2011
The ribosome is critical for all aspects of cell growth due to its essential role in protein synthesis. Paradoxically, many Ribosomal proteins (Rps) act as tumour suppressors in Drosophila and vertebrates. To examine how reductions in Rps could lead to tissue overgrowth, we took advantage of the observation that an RpS6 mutant dominantly suppresses the small rough eye phenotype in a cyclin E hypomorphic mutant (cycE(JP)). We demonstrated that the suppression of cycE(JP) by the RpS6 mutant is not a consequence of restoring CycE protein levels or activity in the eye imaginal tissue. Rather, the use of UAS-RpS6 RNAi transgenics revealed that the suppression of cycE(JP) is exerted via a mechanis..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by NHMRC
This work was supported by grants from the National Health and Medical Research Council (NHMRC) of Australia to RDH (NHMRC grants #251688; #1003270; #509088; #400116, #400114; Senior Research Fellowship #166908), to RBP (NHMRC grant #509087, #400116, # 251688; Senior Research Fellowship #509027), and to LMQ (NHMRC grants #400114; #10044791 and #628414); by the Australian Postgraduate Award to JIL; and by funding from Cancer Research UK to SJM, CDW, and SJL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.