Journal article
γH2AX foci as a measure of DNA damage: A computational approach to automatic analysis
AN Ivashkevich, OA Martin, AJ Smith, CE Redon, WM Bonner, RF Martin, PN Lobachevsky
Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis | Published : 2011
Abstract
The γH2AX focus assay represents a fast and sensitive approach for the detection of one of the critical types of DNA damage - double-strand breaks (DSB) induced by various cytotoxic agents including ionising radiation. Apart from research applications, the assay has a potential in clinical medicine/pathology, such as assessment of individual radiosensitivity, response to cancer therapies, as well as in biodosimetry. Given that generally there is a direct relationship between numbers of microscopically visualised γH2AX foci and DNA DSB in a cell, the number of foci per nucleus represents the most efficient and informative parameter of the assay. Although computational approaches have been dev..
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Awarded by National Institutes of Health
Funding Acknowledgements
The research was supported by a licensing agreement between Sirtex Medical Inc and Peter MacCallum Cancer Centre and by the Intramural Research Program of the National Cancer Institute, National Institutes of Health.