Preclinical characterization of F-18-D-FPHCys, a new amino acid-based PET tracer
Delphine Denoyer, Laura Kirby, Kelly Waldeck, Peter Roselt, Oliver C Neels, Thomas Bourdier, Rachael Shepherd, Andrew Katsifis, Rodney J Hicks
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | SPRINGER | Published : 2012
PURPOSE: The imaging potential of a new (18)F-labelled methionine derivative, S-(3-[(18)F]fluoropropyl)-D-homocysteine ((18)F-D-FPHCys), and its selectivity for amino acid transporter subtypes were investigated in vitro and by imaging of human tumour xenografts. METHODS: Expression of members of the system L (LAT isoforms 1-4 and 4F2hc) and ASCT (ASCT isoforms 1 and 2) amino acid transporter subclasses were assessed by quantitative real-time PCR in four human tumour models, including A431 squamous cell carcinoma, PC3 prostate cancer, and Colo 205 and HT-29 colorectal cancer lines. The first investigations for the characterization of (18)F-D-FPHCys were in vitro uptake studies by comparing it..View full abstract
This study was funded by the Australian Government Cooperative Research Centre for Biomedical Imaging Development Ltd (CRCBID), Bundoora, Victoria, Australia.