Promoter-Specific Expression and Imprint Status of Marsupial IGF2
Jessica M Stringer, Shunsuke Suzuki, Andrew J Pask, Geoff Shaw, Marilyn B Renfree
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2012
In mice and humans, IGF2 has multiple promoters to maintain its complex tissue- and developmental stage-specific imprinting and expression. IGF2 is also imprinted in marsupials, but little is known about its promoter region. In this study, three IGF2 transcripts were isolated from placental and liver samples of the tammar wallaby, Macropus eugenii. Each transcript contained a unique 5' untranslated region, orthologous to the non-coding exons derived from promoters P1-P3 in the human and mouse IGF2 locus. The expression of tammar IGF2 was predominantly from the P2 promoter, similar to humans. Expression of IGF2 was higher in pouch young than in the adult and imprinting was highly tissue and d..View full abstract
This work was supported by the Australian Research Council (ARC) Centre of Excellence in Kangaroo Genomics; an ARC Federation Fellowship to MBR and a National Health and Medical Research Council R.D. Wright Fellowship to AP and the Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad to SS. JMS received an Australian Postgraduate Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.