HIV Infection Abrogates the Functional Advantage of Natural Killer Cells Educated through KIR3DL1/HLA-Bw4 Interactions To Mediate Anti-HIV Antibody-Dependent Cellular Cytotoxicity

Matthew S Parsons; Leia Wren; Gamze Isitman; Marjon Navis; Ivan Stratov; Nicole F Bernard; Stephen J Kent

Journal article

HIV Infection Abrogates the Functional Advantage of Natural Killer Cells Educated through KIR3DL1/HLA-Bw4 Interactions To Mediate Anti-HIV Antibody-Dependent Cellular Cytotoxicity

Matthew S Parsons, Leia Wren, Gamze Isitman, Marjon Navis, Ivan Stratov, Nicole F Bernard, Stephen J Kent

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2012

DOI: 10.1128/JVI.06112-11

Abstract

Combinations of KIR3DL1 and HLA-Bw4 alleles protect against HIV infection and/or disease progression. These combinations enhance NK cell responsiveness through the ontological process of education. However, educated KIR3DL1(+) NK cells do not have enhanced degranulation upon direct recognition of autologous HIV-infected cells. Since antibody-dependent cellular cytotoxicity (ADCC) is associated with improved HIV infection outcomes and NK cells overcome inhibition through killer cell immunoglobulin-like receptors (KIR) to mediate ADCC, we hypothesized that KIR3DL1-educated NK cells mediate anti-HIV ADCC against autologous cells. A whole-blood flow cytometry assay was used to evaluate ADCC-indu..

View full abstract

University of Melbourne Researchers

Stephen Kent Author Microbiology and Immunology

Related Projects (3)

Role of HIV-specific ADCC activity in progression of HIV-1 in humans

DESCRIPTION (provided by applicant): A vaccine for HIV is a global health priority. Efforts to develop a successful HIV vaccine using standa..

Development of purified antibodies that kill virus infected cells

This proposal will develop panels of purified and monoclonal antibodies that kill virus infected cells. These antibodies may show efficacy i..

HIV & HCV Vaccines and Immunopathogenesis

The development of vaccines and better treatments for HIV-AIDS and Hepatitis C are urgent global health priorities. This Program will undert..

Grants

Awarded by National Health and Medical Research Council

Awarded by Australian Research Council

Awarded by National Institutes of Health

Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

Funding Acknowledgements

This work was supported by National Health and Medical Research Council grant 510448, Australian Research Council grant LP0991498, and National Institutes of Health grant R21AI081541 and by the Australian Centre for HIV and Hepatitis Virology Research, the Royal Australasian College of Physicians, and the Ramaciotti Foundation. M.S.P. is supported by a Vanier Scholarship from the Canadian Institutes for Health Research (CIHR) and is a visiting scholar at the University of Melbourne through a Michael Smith Foreign Study Supplement from the CIHR.