Journal article

Arl5b is a Golgi-localised small G protein involved in the regulation of retrograde transport

Fiona J Houghton, Shayne A Bellingham, Andrew F Hill, Dorothee Bourges, Desmond KY Ang, Timothy Gemetzis, Isabelle Gasnereau, Paul A Gleeson

EXPERIMENTAL CELL RESEARCH | ELSEVIER INC | Published : 2012

Abstract

Regulation of membrane transport is controlled by small G proteins, which include members of the Rab and Arf families. Whereas the role of the classic Arf family members are well characterized, many of the Arf-like proteins (Arls) remain poorly defined. Here we show that Arl5a and Arl5b are localised to the trans-Golgi in mammalian cells, and furthermore have identified a role for Arl5b in the regulation of retrograde membrane transport from endosomes to the trans-Golgi network (TGN). The constitutively active Arl5b (Q70L)-GFP mutant was localised efficiently to the Golgi in HeLa cells whereas the dominant-negative Arl5b (T30N)-GFP mutant was dispersed throughout the cytoplasm and resulted i..

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