Journal article
The Wnt receptor Ryk plays a role in mammalian planar cell polarity signaling
ML Macheda, WW Sun, K Kugathasan, BM Hogan, NI Bower, MM Halford, YF Zhang, BE Jacques, GJ Lieschke, A Dabdoub, SA Stacker
Journal of Biological Chemistry | Published : 2012
Abstract
Wnts are essential for a wide range of developmental processes, including cell growth, division, and differentiation. Some of these processes signal via the planar cell polarity (PCP) pathway, which is a β-catenin-independent Wnt signaling pathway. Previous studies have shown that Ryk, a member of the receptor tyrosine kinase family, can bind to Wnts. Ryk is required for normal axon guidance and neuronal differentiation during development. Here, we demonstrate that mammalian Ryk interacts with the Wnt/PCP pathway. In vitro analysis showed that the Wnt inhibitory factor domain of Ryk was necessary for Wnt binding. Detailed analysis of two vertebrate model organisms showed Ryk phenotypes consi..
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Awarded by National Institute on Deafness and Other Communication Disorders
Funding Acknowledgements
This work was supported in part by a program grant (to S. A. S.) from the National Health and Medical Research Council of Australia and Grant R01DC011104 (to A. D.) from the National Institutes of Health.Supported by a Senior Research Fellowship from the National Health and Medical Research Council of Australia and a Pfizer Australia Fellowship. To whom correspondence should be addressed: Peter MacCallum Cancer Centre, Locked Bag 1, A'Beckett St., Melbourne, Victoria 8006, Australia. Tel.: 61-3-9656-5263; Fax: 61-3-9656-1411; E-mail: steven.stacker@petermac.org.We thank R. Moon for the super TOP-FLASH and super FOP-FLASH plasmids; A. McMahon for the Wnt3a and Wnt11 plasmids; R. Habas for the pGEX-RBD plasmid; Y. Zou for the anti-Ryk antibodies; S. Jane for providing the Vangl2<SUP>Lp</SUP> mice; A. Naughton and staff at the Biological Resource Facility at the Ludwig Institute for Cancer Research, Melbourne, Australia, for assistance with mouse experiments; S. Cody for assistance with imaging; S. Paquet-Fifield for assistance with photography; and M. Achen for critical reading of the manuscript. Fluorescent images were generated at the University of California, San Diego, Neuroscience Microscopy Shared Facility supported by National Institutes of Health NS/NINDS HHS Grant P30-NS047101.