Journal article

Local orientational disorder in peptide fibrils probed by a combination of residue-specific C-13-O-18 labelling, polarised infrared spectroscopy and molecular combing

Jose C Rodriguez-Perez, Ian W Hamley, Sally L Gras, Adam M Squires

CHEMICAL COMMUNICATIONS | ROYAL SOC CHEMISTRY | Published : 2012

Abstract

A novel combination of site-specific isotope labelling, polarised infrared spectroscopy and molecular combing reveals local orientational ordering in the fibril-forming peptide YTIAALLSPYSGGRADS. Use of (13)C-(18)O labelled alanine residues demonstrates that the N-terminal end of the peptide is incorporated into the cross-beta structure, while the C-terminal end shows orientational disorder.

University of Melbourne Researchers