Journal article
Myelin gene regulatory factor is required for maintenance of myelin and mature oligodendrocyte identity in the adult CNS
M Koenning, S Jackson, CM Hay, C Faux, TJ Kilpatrick, M Willingham, B Emery
Journal of Neuroscience | Published : 2012
Abstract
Although the transcription factors required for the generation of oligodendrocytes and CNS myelination during development have been relatively well established, it is not known whether continued expression of the same factors is required for the maintenance of myelin in the adult. Here, we use an inducible conditional knock-out strategy to investigate whether continued oligodendrocyte expression of the recently identified transcription factor myelin gene regulatory factor (MRF) is required to maintain the integrity of myelin in the adult CNS. Genetic ablation of MRF in mature oligodendrocytes within the adult CNS resulted in a delayed but severe CNS demyelination, with clinical symptoms begi..
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Awarded by National Eye Institute
Funding Acknowledgements
This work was supported by the Myelin Repair Foundation and grants from the Australian National Health and Medical Research Council (NHMRC Grant 1009095), the Australian Multiple Sclerosis Society, the Trish Multiple Sclerosis Research Foundation, and National Eye Institute Grant EY10257. B. E. is supported by an NHMRC Career Development Fellowship. We thank Brian Popko for kindly providing the PLP-CreERT mouse line and for helpful discussions. We are indebted to Anna Friedhuber for processing samples for TEM and to Ben Barres and David Lyons for constructive criticism of this manuscript during preparation.