Acylation-dependent and-independent membrane targeting and distinct functions of small myristoylated proteins (SMPs) in Leishmania major
Dedreia Tull, Joanne Heng, Paul R Gooley, Thomas Naderer, Malcolm J McConville
INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2012
Trypanosomatid parasites express a number of mono- and diacylated proteins that are targeted to distinct regions of the plasma membrane including the cell body, the flagellum and the flagellar pocket. The extent to which the acylation status and other protein motifs regulate the targeting and/or retention of these proteins to the distinct membrane domains is poorly defined. We have previously described a family of small myristoylated proteins (SMPs) that are either monoacylated (myristoylated) or diacylated (myristoylated and palmitoylated) and targeted to distinct plasma membrane domains. Diacylated SMP-1 is a major constituent of the flagellar membrane, whereas monoacylated SMP-2 resides i..View full abstract
We are indebted to Dr. Timothy Spurck (deceased) for expert assistance with the live cell imaging. We thank Drs. Emanuela Handman (Walter and Eliza Hall Institute, Melbourne, Australia) and Thomas Ilg (Intervet Innovation, GmbH, Schwabenheim, Germany) for generously providing GIPL2, PSA-2 and PMM antibodies and Prof Stephen Beverley (School of Medicine, Washington University, St. Louis, USA) for the pX-plasmids. This work was funded by the National Health and Medical Research Council (NHMRC), Australia. M.J.M. is a NHMRC Principal Research Fellow.