Journal article
Ploidy stability of somatic embryo-derived plants in two ecological keystone sedge species (Lepidosperma laterale and L. concavum, Cyperaceae)
A Kodym, EM Temsch, E Bunn, J Delpratt
Australian Journal of Botany | CSIRO PUBLISHING | Published : 2012
DOI: 10.1071/BT12025
Abstract
We report on the development of a somatic embryogenesis system for Lepidosperma concavum R.Br. and L. laterale R.Br. and the determination of ploidy stability of plants derived from somatic embryos. These keystone Lepidosperma species cannot currently be returned to restoration sites because of propagation difficulties (i.e. seed dormancy, low seed fill and recalcitrance to vegetative propagation). Three explant types (in vitro-germinated seedlings, immature seed and immature inflorescences) were used for the assessment of callus production potential. Embryogenic callus was induced and multiplied on 1/2MS medium with 2,4-D either alone, or in combination with zeatin. Over 90% of seedling exp..
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Funding Acknowledgements
We thank the Royal Botanic Gardens (RBG) Melbourne for the use of the aspirator and the preparation of voucher specimen. Many thanks go to D. Carew for carrying out the rain-garden plantings and R. Dabal for the field plantings and for providing photos and data. Further, W. Worboys from RBG Cranbourne is thanked for enabling seed collections, G. Lorimer for species identification as well as M. Panaia, R. Barrett and J. Greilhuber for fruitful discussions. Special thanks go to K. Vohs and G. Brown for the shipment of plant material to Austria. A. Kodym was an Erwin Schrodinger Fellow from the Austrian Science Fund (Project J2729-BO3). This project was partially supported by Melbourne Water.