Conjugation of Transferrin to Azide-Modified CdSe/ZnS Core-Shell Quantum Dots using Cyclooctyne Click Chemistry
Christine Schieber, Alessandra Bestetti, Jet Phey Lim, Anneke D Ryan, Nguyen Tich-Lam, Robert Eldridge, Anthony R White, Paul A Gleeson, Paul S Donnelly, Spencer J Williams, Paul Mulvaney
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | WILEY-V C H VERLAG GMBH | Published : 2012
Twinkle twinkle quantum dot: Conjugation of biomolecules to azide-modified quantum dots (QDs) through a bifunctional linker, using strain-promoted azide-alkyne cycloaddition with the QD and a squaramide linkage to the biomolecule (see scheme). Transferrin-conjugated QDs were internalized by transferrin-receptor expressing HeLa cells.
This work was supported by the Faculty of Science, University of Melbourne, and the Australian Research Council (ARC).