Transcriptomic profiling of astrocytes treated with the Rho kinase inhibitor Fasudil reveals cytoskeletal and pro-survival responses
Chew L Lau, Victoria M Perreau, Minghui J Chen, Holly S Cate, Daniel Merlo, Nam S Cheung, Ross D O'Shea, Philip M Beart
Journal of Cellular Physiology | WILEY | Published : 2012
Inhibitors of Rho kinase (ROCK) have potential for management of neurological disorders by inhibition of glial scarring. Since astrocytes play key roles in brain physiology and pathology, we determined changes in the astrocytic transcriptome produced by the ROCK inhibitor Fasudil to obtain mechanistic insights into its beneficial action during brain injury. Cultured murine astrocytes were treated with Fasudil (100 µM) and morphological analyses revealed rapid stellation by 1 h and time-dependent (2-24 h) dissipation of F-actin-labelled stress fibres. Microarray analyses were performed on RNA and the time-course of global gene profiling (2, 6, 12 and 24 h) provided a comprehensive description..View full abstract
This study was supported by NH&MRC (Australia), P.M.B. is a Research Fellow of the NH&MRC (Australia), H.S.C. is supported by a William Collie Fellowship (University of Melbourne), N.C.S. is supported by Strategic Initiative Funding (Menzies Research Institute), M.J.C. is supported by an Endeavour Postgraduate Award (Department of Education, Employment and Workplace Relations, Australia).