Journal article
Evaluation of microrna expression in patient bone marrow aspirate slides
L Morenos, R Saffery, F Mechinaud, D Ashley, N Elwood, JM Craig, NC Wong
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Open access
Abstract
Like formalin fixed paraffin embedded (FFPE) tissues, archived bone marrow aspirate slides are an abundant and untapped resource of biospecimens that could enable retrospective molecular studies of disease. Historically, RNA obtained from slides is limited in utility because of their low quality and highly fragmented nature. MicroRNAs are small (≈22 nt) non-coding RNA that regulate gene expression, and are speculated to preserve well in FFPE tissue. Here we investigate the use of archived bone marrow aspirate slides for miRNA expression analysis in paediatric leukaemia. After determining the optimal method of miRNA extraction, we used TaqMan qRT-PCR to identify reference miRNA for normalisat..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the Victorian Cancer Agency Grant; the National Health & Medical Research Council, Australia [Project Grant number 607306]; the Victorian Government's Operational Infrastructure Support Program; the National Health & Medical Research Council Dora Lush Postgraduate Scholarship to LM; and the Leukaemia Foundation of Australia Phillip Desbrow Postdoctoral Fellowship and Project Grant in Aid to NW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.