Opsonization of malaria-infected erythrocytes activates the inflammasome and enhances inflammatory cytokine secretion by human macrophages
Jingling Zhou, Louise E Ludlow, Wina Hasang, Stephen J Rogerson, Anthony Jaworowski
MALARIA JOURNAL | BMC | Published : 2012
BACKGROUND: Antibody opsonization of Plasmodium falciparum-infected erythrocytes (IE) plays a crucial role in anti-malarial immunity by promoting clearance of blood-stage infection by monocytes and macrophages. The effects of phagocytosis of opsonized IE on macrophage pro-inflammatory cytokine responses are poorly understood. METHODS: Phagocytic clearance, cytokine response and intracellular signalling were measured using IFN-γ-primed human monocyte-derived macrophages (MDM) incubated with opsonized and unopsonized trophozoite-stage CS2 IE, a chondroitin sulphate-binding malaria strain. Cytokine secretion was measured by bead array or ELISA, mRNA using quantitative PCR, and activation of NF-..View full abstract
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Awarded by Australian NHMRC
We thank the Australian Red Cross Blood Bank for the provision of human blood and the pregnant women and clinical staff in Malawi for plasma samples. We thank Gaoqian Feng and Francisca Yosaatmadja for help in preparation of trophozoites. This work was supported by Australian NHMRC Project Grants 400090 and 628611 to AJ and SJR. The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program. The funding body had no role in the collection, analysis, and interpretation of data, in the writing of the manuscript, and in the decision to submit the manuscript for publication.