Journal article
High and low mammographic density human breast tissues maintain histological differential in murine tissue engineering chambers
GL Chew, D Huang, SJ Lin, C Huo, T Blick, MA Henderson, P Hill, J Cawson, WA Morrison, IG Campbell, JL Hopper, MC Southey, I Haviv, EW Thompson
Breast Cancer Research and Treatment | Published : 2012
Abstract
Mammographic density (MD) is the area of breast tissue that appears radiologically white on mammography. Although high MD is a strong risk factor for breast cancer, independent of BRCA1/2 mutation status, the molecular basis of high MD and its associated breast cancer risk is poorly understood. MD studies will benefit from an animal model, where hormonal, gene and drug perturbations on MD can be measured in a preclinical context. High and low MD tissues were selectively sampled by stereotactic biopsy from operative specimens of high-risk women undergoing prophylactic mastectomy. The high and low MD tissues were transferred into separate vascularised biochambers in the groins of SCID mice. Ch..
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Funding Acknowledgements
This study was supported by the Victorian Breast Cancer Research Consortium (MCS, IGC, EWT, JH), the St Vincent's Hospital Research Endowment Fund (EWT, JC, PH 2008, 2009), and National Health and Medical Research Council (GLC, MCS, JH, IGC). SJL is supported by the NSS-PhD scholarship from the Agency for Science, Technology, and Research (A*STAR). St Vincent's Institute and The O'Brien Institute receive infrastructure funding from the Victorian State Government under the Medical Research Operational Infrastructure program. We thank Sue MacAuley and Nadine Wood (St Vincent's BreastScreen, St Vincent's Hospital, Victoria) for help with radiography and tissue sampling; The Victorian Cancer Biobank (Peter MacCallum Cancer Centre) for help with tissue accrual; The Department of Pathology, St Vincent's Hospital Melbourne, for assistance with tissue processing and immunohistochemical staining on the autostainer.