Two lines of transgenic mice expressing cre-recombinase exhibit increased seizure susceptibility

Tae Hwan Kim; Kay Richards; Julian Heng; Steven Petrou; Christopher A Reid

Journal article

Two lines of transgenic mice expressing cre-recombinase exhibit increased seizure susceptibility

Tae Hwan Kim, Kay Richards, Julian Heng, Steven Petrou, Christopher A Reid

EPILEPSY RESEARCH | ELSEVIER SCIENCE BV | Published : 2013

DOI: 10.1016/j.eplepsyres.2012.10.005

Abstract

Conditional mouse models based on the Cre-recombinase (Cre)-loxP method are a powerful tool for determining the spatial and temporal function of genes in neuroscience research. The Emx1-Cre conditional model is designed to drive Cre expression in a predominantly excitatory neuron specific manner and the Dlx5/6-Cre mouse expresses Cre predominantly in cortical inhibitory neurons. The mouse models expressing the Cre transgene are healthy, active and have no overt behavioural or brain histological phenotypes. Subcutaneous pentylenetetrazol (scPTZ) is a proconvulsant frequently used to probe neuronal network excitability. In both the Emx1-Cre and Dlx5/6-Cre conditional mouse models the latency t..

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University of Melbourne Researchers

Steven Petrou Author Florey Department of Neuroscience and Mental Health

Christopher Reid Author Medicine - Austin Health

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Grants

Awarded by NHMRC project grant

Awarded by NHMRC program grant

Awarded by ARC Future Fellowship

Funding Acknowledgements

Supported by NHMRC project grant 628520 to (CAR) and a NHMRC program grant 400121 to SP. CAR also acknowledge the support of an ARC Future Fellowship (FT0990628), The Florey Neuroscience Institutes. The Florey Neuroscience Institutes are supported by the Victorian Government.