Journal article
Isolation and Characterization of Tumor Cells from the Ascites of Ovarian Cancer Patients: Molecular Phenotype of Chemoresistant Ovarian Tumors
A Latifi, RB Luwor, M Bilandzic, S Nazaretian, K Stenvers, J Pyman, H Zhu, EW Thompson, MA Quinn, JK Findlay, N Ahmed
Plos One | Published : 2012
Abstract
Tumor cells in ascites are a major source of disease recurrence in ovarian cancer patients. In an attempt to identify and profile the population of ascites cells obtained from ovarian cancer patients, a novel method was developed to separate adherent (AD) and non-adherent (NAD) cells in culture. Twenty-five patients were recruited to this study; 11 chemonaive (CN) and 14 chemoresistant (CR). AD cells from both CN and CR patients exhibited mesenchymal morphology with an antigen profile of mesenchymal stem cells and fibroblasts. Conversely, NAD cells had an epithelial morphology with enhanced expression of cancer antigen 125 (CA125), epithelial cell adhesion molecule (EpCAM) and cytokeratin 7...
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This was supported by Women's Cancer Foundation, National Health and Medical Research Council of Australia (JKF, RegKey#441101), the National Breast Cancer Foundation (EWT; EMPathy Breast Cancer Network, Australia) and the Victorian Government's Operational Infrastructure Support Program (Australia). The funders had no role in study desugn, data collection and analysis, decision to publish or preparation of the manuscript.