Journal article
Improving the quality of electron tomography image volumes using pre-reconstruction filtering
M Maiorca, E Hanssen, E Kazmierczak, B Maco, M Kudryashev, R Hall, H Quiney, L Tilley
Journal of Structural Biology | Published : 2012
Abstract
Electron tomography produces highly magnified 3D image volumes useful for investigating the structure and function of cellular components. Image quality is degraded by multiple scattering events and quantum noise, which depend on the angle at which individual tilt projections are collected. We have adapted a biomedical imaging approach to improve image quality by enhancing individual tilt projections prior to volumetric reconstruction. Specifically, we have developed a family of non-linear anisotropic diffusion (NAD) filters parameterized by the tilt angle. We give a quantitative and qualitative evaluation of our pre-processing approach and the NAD filter. We show an improvement in the recon..
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Funding Acknowledgements
The authors acknowledge support from the Australian Research Council and the Australian National Health and Medical Research Council. We thank Kenneth Downing, Lawrence Berkeley Laboratories, CA, USA and Carlos Oscar Sorzano, Centro Nacional Biotecnologia, Madrid, Spain, and Bernard Heymann, National Institutes of Health, MD, USA, and David Mastronarde, University of Colorado, CO, USA, and Ruben Dilanian, University of Melbourne, Australia, for helpful discussions. We thank Freddy Frischknecht and Marek Cyrklaff (University of Heidelberg, Germany) and Wolfgang Baumeister (Max Planck for Biochemistry, Munich, Germany) for support in cryo data acquisition. The photo of Marie Curie is from the nobelprize.org website.