Journal article

Drosophila Rbp6 Is an Orthologue of Vertebrate Msi-1 and Msi-2, but Does Not Function Redundantly with dMsi to Regulate Germline Stem Cell Behaviour

Nicole A Siddall, Marina Kalcina, Timothy M Johanson, Adrian C Monk, Franca Casagranda, Reeva P Been, Eileen A McLaughlin, Gary R Hime



The vertebrate RNA-binding proteins, Musashi-1 (Msi-1) and Musashi-2 (Msi-2) are expressed in multiple stem cell populations. A role for Musashi proteins in preventing stem cell differentiation has been suggested from genetic analysis of the Drosophila family member, dMsi, and both vertebrate Msi proteins function co-operatively to regulate neural stem cell behaviour. Here we have identified a second Drosophila Msi family member, Rbp6, which shares more amino acid identity with vertebrate Msi-1 and Msi-2 than dMsi. We generated an antibody that detects most Rbp6 splice isoforms and show that Rbp6 is expressed in multiple tissues throughout development. However, Rbp6 deletion mutants generate..

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Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was funded by the Australian Research Council Centre of Excellence in Biotechnology and Development,, and the National Health and Medical Research Council Project Grant No. 569234, The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.