GAL4 Enhancer Traps That Can Be Used to Drive Gene Expression in Developing Drosophila Spermatocytes
Stephanie M Bunt, Adrian C Monk, Nicole A Siddall, Neisha L Johnston, Gary R Hime
GENESIS | WILEY | Published : 2012
The Drosophila testis has proven to be a valuable model organ for investigation of germline stem cell (GSC) maintenance and differentiation as well as elucidation of the genetic programs that regulate differentiation of daughter spermatogonia. Development of germ cell specific GAL4 driver transgenes has facilitated investigation of gene function in GSCs and spermatogonia but specific GAL4 tools are not available for analysis of postmitotic spermatogonial differentiation into spermatocytes. We have screened publically available pGT1 strains, a GAL4-encoding gene trap collection, to identify lines that can drive gene expression in late spermatogonia and early spermatocytes. While we were unabl..View full abstract
Awarded by Discovery Project
The authors would like to thank Coral Warr, Australian Drosophila Biomedical Research Support Facility, Bloomington Drosophila Stock Center, and Kyoto Drosophila Genetic Resource Center for provision of Drosophila strains and the Developmental Studies Hybridoma Bank for antibodies. This study was supported by the Australian Research Council Centre of Excellence in Biotechnology and Development and Discovery Project 120100224.