A proline repeat polymorphism of the Frost gene of Drosophila melanogaster showing clinal variation but not associated with cold resistance
AA Hoffmann, MJ Blacket, SW McKechnie, L Rako, M Schiffer, RV Rane, RT Good, C Robin, SF Lee
INSECT MOLECULAR BIOLOGY | WILEY | Published : 2012
Genetic polymorphisms underlying adaptive shifts in thermal responses are poorly known even though studies are providing a detailed understanding of these responses at the cellular and physiological levels. The Frost gene of Drosophila melanogaster is a prime candidate for thermal adaptation; it is up-regulated under cold stress and knockdown of this gene influences cold resistance. Here we describe an amino-acid INDEL polymorphism in proline repeat number in the structural component of this gene. The two main repeats, accounting for more than 90% of alleles in eastern Australia, show a strong clinal pattern; the 6P allele was at a high frequency in tropical locations, and the 10P allele was..View full abstract
We thank Jennifer Shirriffs, Alen Rako, Alisha Anderson and Rebecca Hallas for laboratory assistance as well as Jessica Fielding for preliminary data. This work was supported by Discovery and Fellowship grants from the Australian Research Council.