Journal article

Revascularization and tissue regeneration of an empty root canal space is enhanced by a direct blood supply and stem cells

Tanida Srisuwan, Daniel J Tilkorn, Sammy Al-Benna, Keren Abberton, Harold H Messer, Erik W Thompson



BACKGROUND: Regenerative endodontics is an innovative treatment concept aiming to regenerate pulp, dentin and root structures. In the diseased or necrotic tooth, the limitation in vascular supply renders successful tissue regeneration/generation in a whole tooth challenging. The aim of this study is to evaluate the ability of vascularized tissue to develop within a pulpless tooth using tissue engineering techniques. MATERIALS AND METHODS: A pulpless tooth chamber, filled with collagen I gel containing isolated rat dental pulp cells (DPC) and angiogenic growth factors, was placed into a hole created in the femoral cortex or into its own tooth socket, respectively. The gross, histological and ..

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Funding Acknowledgements

This investigation was supported in part by Australian Dental Research Foundation and the Australian Society of Endodontology, Australia. We gratefully acknowledge Mr. Aditya Vashi for preparing gelatin microspheres, all staff of the Experimental Medical and Surgical Unit, St Vincent's Hospital, Melbourne for providing animal facilities, the overall support of Professor Wayne Morrison, Bernard O'Brien Institute of Medical Research, and the general support and interaction with members of the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Australia.