Journal article

CLONING AND CHARACTERIZATION OF CUTE, A GENE INVOLVED IN COPPER TRANSPORT IN ESCHERICHIA-COLI

SD ROGERS, MR BHAVE, JFB MERCER, J CAMAKARIS, BTO LEE

JOURNAL OF BACTERIOLOGY | AMER SOC MICROBIOLOGY | Published : 1991

Abstract

The copper-sensitive/temperature-sensitive phenotype of the Escherichia coli cutE mutant has been complemented by cloning wild-type genomic DNA into the plasmid vector pACYC184 and selecting transformants on medium containing 4 mM copper sulfate and chloramphenicol. One of these complementing clones, designated pCUT1, contained a 5.6-kb BamHI fragment. This recombinant plasmid transformed cutE, allowing wild-type growth of transformants on medium containing copper sulfate. Complementation of copper sensitivity was assessed by comparing both cell survival at increased copper levels and the results of 64Cu accumulation assays. An EcoRI subclone, 2.3 kb in size, was also shown to complement cut..

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University of Melbourne Researchers