Shape-Dependent Cellular Processing of Polyelectrolyte Capsules

Olga Shimoni; Yan Yan; Yajun Wang; Frank Caruso

Journal article

Shape-Dependent Cellular Processing of Polyelectrolyte Capsules

Olga Shimoni, Yan Yan, Yajun Wang, Frank Caruso

ACS NANO | AMER CHEMICAL SOC | Published : 2013

DOI: 10.1021/nn3046117

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Abstract

Particle shape is emerging as a key design parameter for tailoring the interactions between particles and cells. Herein, we report the preparation of rod-shaped layer-by-layer (LbL)-assembled polymer hydrogel capsules with tunable aspect ratios (ARs). By templating spherical and rodlike silica particles, disulfide-stabilized poly(methacrylic acid) hydrogel capsules (PMA HCs) with different ARs (from 1 to 4) are generated. The influence of capsule AR on cellular internalization and intracellular fate was quantitatively investigated by flow cytometry, imaging flow cytometry, and fluorescence deconvolution microscopy. These experiments reveal that the cellular internalization kinetics of PMA HC..

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University of Melbourne Researchers

Frank Caruso Author Chemical Engineering

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

This work was supported by the Australian Research Council under the Australian Laureate Fellowship (F.C.), Federation Fellowship (F.C.), and Discovery Project (F.C.) schemes, and by the National Health and Medical Research Council (NHMRC) Program Grant 487922 (F.C.). James P. Best is acknowledged for helpful discussions on preparation of the manuscript. Kang Liang is acknowledged for assistance with the AFM measurements.