Microbeam-irradiated tumour tissue possesses a different infrared absorbance profile compared to broad beam and sham-irradiated tissue

Abstract

Purpose: To investigate biochemical changes in mouse tumour tissue following Microbeam Radiation Therapy (MRT) and Broad Beam (BB) irradiation using synchrotron Fourier-Transform Infrared (FTIR) microspectroscopy. Materials and methods: Synchrotron FTIR microspectroscopy was carried out on mouse tumour sections previously irradiated with BB (11, 22 or 44 Gy), MRT (560 Gy in-beam, 25 μm wide, 200 μm peak separation) or sham-irradiation (0 Gy) from mice culled 4 hours post-irradiation. Results: MRT and BB-irradiated tumour sections showed clear chemical shifts in spectral bands corresponding to functional group vibrations in protein (16541630 cm-1), lipid (~1470, 1463 cm -1) and nucleic acid (..