Isolation and characterization of 10 MHC Class I-associated microsatellite loci in tammar wallaby (Macropus eugenii)
Yuanyuan Cheng, Matthew Wakefield, Hannah V Siddle, Penny C Coggill, Cathy A Herbert, Stephan Beck, Katherine Belov, Mark DB Eldridge
MOLECULAR ECOLOGY RESOURCES | WILEY | Published : 2009
The major histocompatibility complex (MHC) contain genes which play a key role in immune response and mate choice, and are therefore of functional importance to molecular ecologists. Here we describe the design of 10 MHC Class I-associated microsatellite loci from the tammar wallaby. All 10 loci are highly polymorphic, with the expected heterozygosity ranging from 0.547 to 0.919. Six loci successfully cross-amplify in other macropodid species. These microsatellites will serve as useful tools for studying the level of MHC diversity, the impact of selection on genetic variation and the unique structure of the tammar wallaby MHC.
The authors acknowledge project funding from the Australian Research Council to K.B., M.D.B.E. and S.B., sequencing funding from the Wellcome Trust to SB, and wallaby upkeep funding from the ARC Centre of Excellence for Kangaroo Genomics. We sincerely thank James Cook for wallaby upkeep and assistance with sample collection, Lee Miles and Gwilym Haynes for advice on genotyping, Emily Miller for providing the eastern grey kangaroo DNA samples, and Melanie Booth for technical assistance.