Safety and vaccine efficacy of a glycoprotein G deficient strain of infectious laryngotracheitis virus delivered in ovo
Alistair R Legione, Mauricio JC Coppo, Sang-Won Lee, Amir H Noormohammadi, Carol A Hartley, Glenn F Browning, James R Gilkerson, Denise O'Rourke, Joanne M Devlin
Vaccine | ELSEVIER SCI LTD | Published : 2012
Infectious laryngotracheitis virus (ILTV), an alphaherpesvirus, causes respiratory disease in chickens and is commonly controlled by vaccination with conventionally attenuated vaccines. Glycoprotein G (gG) is a virulence factor in ILTV and a gG deficient strain of ILTV (ΔgG-ILTV) has shown potential for use as a vaccine. In the poultry industry vaccination via drinking water is common, but technology is now available to allow quicker and more accurate in ovo vaccination of embryos at 18 days of incubation. In this study ΔgG-ILTV was delivered to chicken embryos at three different doses (10(2), 10(3) and 10(4) plaque forming units per egg) using manual in ovo vaccination. At 20 days after hat..View full abstract
The authors gratefully acknowledge the technical assistance of Cheryl Colson, June Daly and Jenece Wheeler. This research was conducted within the Poultry CRC, established and supported under the Australian Government's Cooperative Research Centres Program. Mauricio Coppo is supported by a Melbourne International Research Scholarship from The University of Melbourne. Joanne Devlin is supported by a Postdoctoral Research Fellowship from the Australian Research Council.