Safety and vaccine efficacy of a glycoprotein G deficient strain of infectious laryngotracheitis virus delivered in ovo

AR Legione; MJC Coppo; SW Lee; AH Noormohammadi; CA Hartley; GF Browning; JR Gilkerson; D O'Rourke; JM Devlin

Journal article

Safety and vaccine efficacy of a glycoprotein G deficient strain of infectious laryngotracheitis virus delivered in ovo

AR Legione, MJC Coppo, SW Lee, AH Noormohammadi, CA Hartley, GF Browning, JR Gilkerson, D O'Rourke, JM Devlin

Vaccine | ELSEVIER SCI LTD | Published : 2012

DOI: 10.1016/j.vaccine.2012.10.023

Abstract

Infectious laryngotracheitis virus (ILTV), an alphaherpesvirus, causes respiratory disease in chickens and is commonly controlled by vaccination with conventionally attenuated vaccines. Glycoprotein G (gG) is a virulence factor in ILTV and a gG deficient strain of ILTV (ΔgG-ILTV) has shown potential for use as a vaccine. In the poultry industry vaccination via drinking water is common, but technology is now available to allow quicker and more accurate in ovo vaccination of embryos at 18 days of incubation. In this study ΔgG-ILTV was delivered to chicken embryos at three different doses (102, 103 and 104 plaque forming units per egg) using manual in ovo vaccination. At 20 days after hatching,..

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University of Melbourne Researchers

Alistair Legione Author

Mauricio Coppo Author

Amir Hadjinoormohammadi Author

Carol Hartley Author

Grants

Funding Acknowledgements

The authors gratefully acknowledge the technical assistance of Cheryl Colson, June Daly and Jenece Wheeler. This research was conducted within the Poultry CRC, established and supported under the Australian Government's Cooperative Research Centres Program. Mauricio Coppo is supported by a Melbourne International Research Scholarship from The University of Melbourne. Joanne Devlin is supported by a Postdoctoral Research Fellowship from the Australian Research Council.