Multiphoton fluorescence lifetime imaging shows spatial segregation of secondary metabolites in Eucalyptus secretory cavities
AM Heskes, CN Lincoln, JQD Goodger, IE Woodrow, TA Smith
Journal of Microscopy - Oxford | WILEY-BLACKWELL | Published : 2012
Multiphoton fluorescence lifetime imaging provides an excellent tool for imaging deep within plant tissues while providing a means to distinguish between fluorophores with high spatial and temporal resolution. Ideal candidates for the application of multiphoton fluorescence lifetime imaging to plants are the embedded secretory cavities found in numerous species because they house complex mixtures of secondary metabolites within extracellular lumina. Previous investigations of this type of structure have been restricted by the use of sectioned material resulting in the loss of lumen contents and often disorganization of the delicate secretory cells; thus it is not known if there is spatial se..View full abstract
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Awarded by Australian Research Council
A. M. H. was supported by the Holsworth Wildlife Research Endowment (managed by ANZ Trustees). J. Q. D. G. and I. E. W. were supported by a grant from the Australian Research Council (Project DP1094530). T. A. S. and C. N. L. acknowledge the support of the Australian Research Council Centre of Excellence for Coherent X-Ray Science. We also acknowledge the assistance of Dr. Lachlan McKimmie in the nonlinear dependence determination.